Which statements are true regarding phage typing? Select all that apply.

A. It is used to characterize and identify strains within a bacterial species.
B. A bacterial strain is a susceptible host for a virus if there is no clearing.
C. Clear zones in the bacterial lawn show that all host cells in that region have been destroyed.
D. If a bacterial host is susceptible to one virus, it will be susceptible to all viruses.
E. During bacteria streaking on a plate that has a phage line down the center, one can cross the line of phage twice.

Phage typing is a method used to identify and characterize bacterial strains based on their susceptibility to specific bacteriophages (viruses that infect bacteria). Clear zones, also known as plaques, in the bacterial lawn indicate that the bacteriophages have destroyed the bacterial cells in that region.

Bacterial hosts susceptible to one phage are likely to be susceptible to other phages as well, due to their similar receptor proteins on the bacterial cell surface. However, it is important to note that not all bacterial strains may be susceptible to all phages, as there may be variations in receptor proteins among different bacterial strains.

Additionally, during bacteria streaking on a plate with a phage line down the center, crossing the line of phage twice can result in mixing of phages, potentially leading to inaccurate results in phage typing.

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