Answer :
Final answer:
The Kirby-Bauer technique is a disk diffusion test measuring antibacterial drugs susceptibility of a bacterial strain. The effectiveness of a drug is indicated by a clear zone of inhibition around a drug-impregnated disk. The size of this zone, compared to a chart, determines if a bacteria is susceptible or resistant to a drug.
Explanation:
The Kirby-Bauer technique, also known as the disk diffusion test, measures the susceptibility of a particular bacterial strain to different antibacterial drugs. The method involves placing drug-impregnated disks on an agar plate that is inoculated with the bacterial strain in question. As the bacteria grow, the antibiotic diffuses from the disk into the agar and interacts with the growing bacteria. The effectiveness of an antimicrobial drug is seen as a clear zone of inhibition around the disk.
Various factors such as the solubility of the drug, the rate of diffusion through the agar, and the concentration of the drug in the disk can influence the size of the zone of inhibition. By comparing the diameter of these zones to a standardized chart, the susceptibility or resistance of the bacterial pathogen to the drug can be determined. It's important to note that the Kirby-Bauer method can't distinguish between bacteriostatic and bactericidal activities, and can't be used to compare drug potencies.
Learn more about Kirby-Bauer Technique here:
https://brainly.com/question/30397203
#SPJ11
