Answer :
Final answer:
Mapping bacterial genes by conjugation is primarily based on the assumption that genes are transferred from donor to recipient in a linear fashion. Other statements about F- and Hfr strains, tolerance to sodium azide, or blender mixing conditions are not the fundamental assumptions of this method.
Explanation:
The concept of mapping bacterial genes by conjugation is predominantly based on the assumption that genes are transferred from a donor to the recipient in a linear fashion. This process, often visualized as a 'rolling circle' model, is propagated through conjugation where one bacterial cell (donor) transfers genetic material to another bacterial cell (recipient). This assumption was addressed by option a. Genes are transferred from donor to recipient in a linear fashion.
F- bacterial strains transferring genes to Hfr strains (option b), the ability of bacterial cells to grow in the presence of sodium azide (option c), or the sustaining of conjugation when a bacterial culture is mixed in a blender (option d) are not the primary assumptions upon which bacterial gene mapping by conjugation is based.
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