Which of the following statements about Hfr strains of E. coli is FALSE?

A. In Hfr strains, the F factor is integrated into a plasmid.
B. Genetic exchange between Hfr and F- strains is studied using the interrupted mating technique.
C. In Hfr strains, the F factor is integrated into the main bacterial chromosome.
D. Hfr stands for "high frequency recombination."
E. The location and order of genes along the chromosome can be mapped using the time of transfer of different marker genes.

The incorrect statement about Hfr strains of E.coli is 'In Hfr strains, the F factor is integrated into a plasmid'. In fact, the F factor is integrated into the main bacterial chromosome, which causes the high frequency of recombination.

Explanation:

The false statement among the ones provided concerning Hfr strains of E. coli is 'In Hfr strains, the F factor is integrated into a plasmid'. This is incorrect because in Hfr strains or High Frequency Recombination strains of E. coli, the F factor is indeed integrated, but into the main bacterial chromosome, not into a plasmid. It's this F factor integration into the main chromosome that allows for the high frequency of recombination.

The interrupted mating technique is accurately described for studying genetic exchange between Hfr and F- strains. The concept of gene mapping using the time of transfer of different marker genes is also correct.

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