High School

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------------------------------------------------ Urine samples should be centrifuged at a:

A. Low speed (1000 to 3000 rpm) for 20 minutes
B. High speed (6000 to 10,000 rpm) for 20 minutes
C. Low speed (1000 to 3000 rpm) for 5 minutes
D. High speed (6000 to 10,000 rpm) for 5 minutes

Answer :

Final answer:

Urine samples should typically be centrifuged at a low speed of 1000 to 3000 rpm for a short duration, often 5 to 10 minutes. This method aligns with practices across other delicate biological samples, aiming for effective sedimentation without damaging components. The correct option is (c) Low speed (1000 to 3000 rpm) for 5 minutes .

Explanation:

The correct method for centrifuging urine samples is typically at a low speed, such as 1000 to 3000 rpm, for a short duration. While laboratory protocols can vary, a common practice is to centrifuge urine at low speeds for around 5 to 10 minutes to allow for efficient sedimentation without causing damage to cellular components that may be present in the urine. This approach helps to sediment the cells and other particulates, allowing for clearer supernatant and better microscopic examination of the sediment. Findings from various procedures involving centrifugation, such as DNA extraction or sample preparation for biochemical analysis, support the use of lower speeds for a shorter duration for delicate samples like urine.

Based on this information, low speed centrifugation of urine samples would be more appropriate than high speed, aligning with other laboratory centrifugation processes where gentle handling of samples is important. The recommended duration, however, may vary depending on the specific protocol of a lab or the analysis being carried out on the urine sample. Nonetheless, option C (Low speed (1000 to 3000 rpm) for 5 minutes) would be the most likely answer given the general practices of urine sediment preparation.