High School

To express the L1 capsid protein in yeast cells, which of the following steps should be taken?

A. Clone the L1 coding sequence downstream of a bacterial promoter.
B. Clone the open reading frame of L1 upstream of a yeast promoter in an expression vector.
C. Clone the entire L1 gene into an expression vector.
D. Clone the L1 coding sequence downstream of a yeast promoter.
E. Clone the HPV genome into an expression vector.

Answer :

Final answer:

The process of expressing the HPV L1 capsid protein in a yeast cell expression system involves cloning the L1 coding sequence into an expression vector and introducing it into yeast cells. The yeast cells then use their own cellular machinery to produce the L1 capsid protein, which is purified and used to make HPV vaccines.

Explanation:

The process of expressing the HPV L1 capsid protein in a yeast cell expression system involves several steps:

  1. Cloning the L1 coding sequence into an expression vector: The L1 coding sequence, which contains the instructions for producing the L1 capsid protein, is cloned into an expression vector. The expression vector is a DNA molecule that can be easily manipulated and introduced into cells.
  2. Introduction of the expression vector into yeast cells: The expression vector, containing the L1 coding sequence, is introduced into yeast cells. This can be done by a process called transformation, where the yeast cells take up the foreign DNA.
  3. Expression of the L1 capsid protein: Once inside the yeast cells, the L1 coding sequence is transcribed into mRNA and then translated into the L1 capsid protein. The yeast cells use their own cellular machinery to carry out this process.
  4. Production of HPV vaccines: The L1 capsid protein produced by the yeast cells is purified and used to make HPV vaccines. The vaccines contain the L1 protein, which can induce an immune response in the body and protect against HPV infection.

Overall, the expression of the HPV L1 capsid protein in a yeast cell expression system is a crucial step in the production of HPV vaccines.

Learn more about expression of hpv l1 capsid protein in a yeast cell expression system here:

https://brainly.com/question/32578209

#SPJ14

Final answer:

The process of expressing the HPV L1 capsid protein in a yeast cell expression system involves cloning the L1 coding sequence into an expression vector and introducing it into yeast cells. The yeast cells then use their own cellular machinery to produce the L1 capsid protein, which is purified and used to make HPV vaccines.

Explanation:

The process of expressing the HPV L1 capsid protein in a yeast cell expression system involves several steps:

  1. Cloning the L1 coding sequence into an expression vector: The L1 coding sequence, which contains the instructions for producing the L1 capsid protein, is cloned into an expression vector. The expression vector is a DNA molecule that can be easily manipulated and introduced into cells.
  2. Introduction of the expression vector into yeast cells: The expression vector, containing the L1 coding sequence, is introduced into yeast cells. This can be done by a process called transformation, where the yeast cells take up the foreign DNA.
  3. Expression of the L1 capsid protein: Once inside the yeast cells, the L1 coding sequence is transcribed into mRNA and then translated into the L1 capsid protein. The yeast cells use their own cellular machinery to carry out this process.
  4. Production of HPV vaccines: The L1 capsid protein produced by the yeast cells is purified and used to make HPV vaccines. The vaccines contain the L1 protein, which can induce an immune response in the body and protect against HPV infection.

Overall, the expression of the HPV L1 capsid protein in a yeast cell expression system is a crucial step in the production of HPV vaccines.

Learn more about expression of hpv l1 capsid protein in a yeast cell expression system here:

https://brainly.com/question/32578209

#SPJ14

Other Questions