Answer :
Ergosterol assay was more sensitive in measuring the early growth of the fungal partner.
The assay is a quantitative method used the determination the purity of metals. One of the simplified processes for fungal ergosterol estimation in ectomycorrhizas is the ergosterol assay. Ethanol and ergosterol are required for this method. By reversed-phase high-performance liquid chromatography, ergosterol and ethanol are quantified.
This process measures the levels of ergo sterol in the fungal biomass. This allows us to estimate fungal infection to a few ectomycorrhizae. Along with this chitin, the assay was prepared for comparison. In measuring ectomycorrhizas, the chitin assay is slow and less convenient as compared to the ergosterol assay. It was observed that the ergosterol assay is rapid and sensitive in measuring fungal early growth.
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