Answer :
Final answer:
The tripeptides will be eluted in the order: TED; DAN; RAY.
Explanation:
Anion exchange chromatography separates molecules based on their net charge. In this case, the tripeptides RAY, DAN, and TED are being analyzed. The order of elution depends on the net charge of each tripeptide at pH 7.0.
At pH 7.0, the carboxylic acid group (COOH) of aspartic acid (D) is deprotonated, giving it a net charge of -1. The amino group (NH2) of lysine (K) is also deprotonated, giving it a net charge of +1. The carboxylic acid group (COOH) of glutamic acid (E) is deprotonated, giving it a net charge of -1.
The order of elution will be based on increasing net negative charge since anion exchange chromatography retains more negatively charged molecules for a longer time. Here's how the tripeptides are charged at pH 7.0:
Based on the net charges, the order of elution will be:
RAY; TED; DAN
Therefore, the tripeptides will be eluted in the order: TED; DAN; RAY
Learn more about tripeptides
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