Answer :
The statement is false. The correct process for a bacteriophage sensitivity plate is to first streak the bacterial strain to form a lawn and then apply the phage, not the other way around. The answer to the question is therefore False.
When conducting a bacteriophage sensitivity plate, you typically streak the bacterial strain onto the plate first to create a bacterial lawn. After that, the bacteriophage (or phage) is applied to the surface of the agar where the bacteria have been plated.
This sequence ensures that the phage can be evenly distributed over the surface of the already established bacterial lawn. In the titering of recombinant phage clones, serial dilutions of a phage lysate are spread across such a bacterial lawn to determine the amount of virus particles present. As the phage infects the bacteria and replicates, clear plaques will form where the bacteria has been lysed.
