High School

What piece of evidence suggested that the lacI gene product must be a repressor?

A. In F' bacterial strains, wildtype versions of the lacI and lacZ genes could both be carried on the F' plasmid.

B. In the presence of the lacI gene product, synthesis of genes in the lac operon was prevented.

C. In F' bacterial strains, the wildtype version of the lacI gene was dominant in both the cis and trans positions.

Answer :

b. In the presence of the lacl gene product, synthesis of genes in the lac operon were prevented suggested that the lacl gene product must be a repressor.

This evidence suggested that the lacI gene product must be a repressor because when the lacI repressor protein binds to the operator region of the lac operon, it blocks the binding of RNA polymerase to the promoter.


This effectively prevents the transcription of the operon's genes, thereby halting the synthesis of the proteins necessary for lactose metabolism in the absence of an inducer like lactose or allolactose.