High School

What basic steps were followed to produce Dolly?

A. Cloning using somatic cell nuclear transfer
B. Hybridization of two different species
C. Artificial insemination
D. Spontaneous generation

Answer :

Final answer:

Dolly the sheep was cloned using a method called somatic cell nuclear transfer. This involved removing the nucleus from a sheep's egg cell and replacing it with the nucleus of a somatic cell from another sheep. The cell was then allowed to divide and was implanted into a surrogate mother.

Explanation:

The basic steps to produce Dolly the sheep were related to a method known as cloning using somatic cell nuclear transfer. This cloning process involves several important steps. Firstly, an egg cell was taken from one sheep, and the nucleus, which contains most of the DNA, was removed.

Then, a nucleus from a somatic cell (body cell), of a second sheep was introduced into this egg cell. The resultant cell, now hosting the DNA of the second sheep, was allowed to divide to an early stage of development known as the blastocyst stage. This cell was then implanted into a surrogate mother sheep and allowed to develop into a new individual - Dolly.

It's noteworthy that this approach does not involve the hybridization of two different species, artificial insemination or spontaneous generation. It took numerous attempts before this process was completed successfully, emphasizing the complex nature of the procedure.

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