High School

Multiple choice questions: pick one.

1.1 The polymerase chain reaction does not require.

a dTNPs

b primers

c DNA polymerase

d DNA ligase

1.2 During your PCR experiment, the temperature used were; pick one under each

1.2.1 Denaturation, Extension, Annealing

- 54 C

- 72 C

- 95 C

1.3 In establishing paternity, how many short tandem repeat (STR) alleles in a child come from the father

a. 1/2

b 3/4

c 1/4

d none

1. 4 Which of these is the correct way to calculate Tm

a. Tm= 4C x (# of GC in primer)/2C x ( # of AT in primer)

b. Tm= 4C x (# of AT in primer ) + 2C x (# of GC in primer)

c. Tm= 41C + 64.9C x (# of GC in primer -16.4) N

d. Tm= 64.9 C + 41 C x (# of GC in primer)

1.5 The polymerase used for PCR is called what and it is extracted from?

1.6 In single PCR cycle consisting of 30 seconds at 95C, 1 min at 55 C, and 1 min at 72C, what is happening in the step run at 55C

a. The DNA to be amplified is being denatured.

b. Primers are annealing to the DNA to be amplified.

c. DNA polymerase is extending new DNA from the primers.

d. Primers are being denatured.

1.7 What is the difference between a restriction endonuclease recognition site and RFLP?

a. Restriction endonuclease recognition sites produce DNA fragments that differ in size when the source of the DNA is from different chromosomes; RFLPs are any sequence of DNA recognized by a restriction endonuclease

b. Restriction endonuclease recognition sites produce DNA fragments that differ in size when the source of the DNA is from different chromosomes; RFLPs are any sequence of RNA amplified by reverse transcriptase and PCR.

c. Restriction endonuclease recognition sites produce DNA fragments that differ in size when the source of the DNA is from different chromosomes; RFLPs are any sequence of DNA recognized by a restriction endonuclease

d. Restriction endonuclease recognition sites are any sequence of DNA recognition endonuclease; RFLPs are DNA fragments produced by restriction endonuclease digestion that differ in size when DNA sequences differ between chromosomes or individuals.

Answer :

Final answer:

The correct answers for the multiple-choice questions are as follows:

  1. 1.1: b) primers
  2. 1.2: Denaturation: 95°C, Extension: 72°C, Annealing: 54°C
  3. 1.3: c) 1/4
  4. 1.4: c) Tm= 41C + 64.9C x (# of GC in primer -16.4) N
  5. 1.5: The polymerase used for PCR is called Taq polymerase, and it is extracted from the thermophilic bacterium Thermus aquaticus.
  6. 1.6: b) Primers are annealing to the DNA to be amplified.
  7. 1.7: d) Restriction endonuclease recognition sites are any sequence of DNA recognized by a restriction endonuclease; RFLPs are DNA fragments produced by restriction endonuclease digestion that differ in size when DNA sequences differ between chromosomes or individuals.

Explanation:

1.1 The polymerase chain reaction does not require: b) primers

1.2 During your PCR experiment, the temperature used were:

  • 1.2.1 Denaturation: 95°C
  • Extension: 72°C
  • Annealing: 54°C

1.3 In establishing paternity, how many short tandem repeat (STR) alleles in a child come from the father: c) 1/4

1.4 Which of these is the correct way to calculate Tm: c) Tm= 41C + 64.9C x (# of GC in primer -16.4) N

1.5 The polymerase used for PCR is called what and it is extracted from: The polymerase used for PCR is called Taq polymerase, and it is extracted from the thermophilic bacterium Thermus aquaticus.

1.6 In a single PCR cycle consisting of 30 seconds at 95°C, 1 minute at 55°C, and 1 minute at 72°C, what is happening in the step run at 55°C: b) Primers are annealing to the DNA to be amplified.

1.7 What is the difference between a restriction endonuclease recognition site and RFLP: d) Restriction endonuclease recognition sites are any sequence of DNA recognized by a restriction endonuclease; RFLPs are DNA fragments produced by restriction endonuclease digestion that differ in size when DNA sequences differ between chromosomes or individuals.

Learn more about pcr (polymerase chain reaction) here:

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