Answer :
Final answer:
A pen should not be used to draw a line on the filter paper in paper chromatography because the ink can interfere with the separation process. Therefore, it's better to use a pencil, as the graphite will not react or interfere.
Explanation:
In paper chromatography, a line is often drawn on the filter paper as a reference point. However, it is not recommended to use a pen to draw this line.
This is because the ink from the pen could dissolve in the solvent and interfere with the separation process of the compounds that you're studying. Instead, utilizing a pencil is more appropriate as graphite, which is non-polar and relatively inert, will not react or interfere in the process.
In a nutshell, the chemicals in pen ink could result in inaccurate results, negatively impacting your experimental accuracy.
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