Answer :
Final answer:
Equine intrauterine insemination involves collecting semen, evaluating its quality, and either directly inseminating or cryopreserving for future use. If frozen, semen is thawed before insemination.The correct option is (b) Semen collection, evaluation, insemination)
Explanation:
The steps in equine intrauterine insemination begin with semen collection, followed by the evaluation of semen to assess its quality. If the semen is to be used immediately, it can then be diluted and inseminated into the mare. However, if the semen is to be used at a later time, it undergoes cryopreservation (freezing). Prior to insemination, the frozen semen would need to be properly thawed. Semen thawing is a critical process which involves melting the frozen semen and bringing it back to body temperature (approximately 37°C) without harming the sperm cells. Once thawed, it can then be used for insemination. So, the correct sequence of steps would be either 'Semen collection, evaluation, insemination' for fresh semen or 'Semen collection, freezing, thawing, insemination' for frozen semen.
