Answer :
Bacterial strains that have mutations preventing them from growing on minimal media without additional supplements are called auxotrophic mutants.
Auxotrophic mutants are bacterial strains that have acquired mutations in specific genes, resulting in the loss of the ability to synthesize certain essential nutrients or molecules required for growth.
As a result, these mutants are unable to grow on minimal media, which only contains the basic nutrients necessary for bacterial growth.
In contrast to wild-type bacteria, which can synthesize all essential nutrients from simple carbon and nitrogen sources present in minimal media, auxotrophic mutants require additional supplements or specific nutrients to support their growth.
These supplements may include specific amino acids, vitamins, or other metabolites that the mutant strain is unable to produce on its own.
Auxotrophic mutants are widely used in genetic and biochemical studies as they provide a means to selectively grow and study specific metabolic pathways or the effects of mutations in certain genes.
By providing the required supplements, researchers can rescue the growth of auxotrophic mutants and investigate the function and regulation of the affected genes.
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